1. Introduction
Stabilizers are substances or chemicals which enable the maintenance of a homogenous dispersion of two or more immiscible substances in a foodstuff and include also substances which stabilize, retain or intensify an existing colour of a foodstuff.
Food stabilizers increases the stability and thickness of the food by binding its large molecules.
Stabilizers are used to increase mix viscosity, improve air incorporation, give body and texture, slow ice crystals formation, control meltdown and interact with proteins.
Emulsifiers are molecules with one hydrophilic and hydrophobic end. Food emulsifiers are also called emulgents. Emulsifiers are substances which make it possible to form or maintain a homogenous mixture of two or more immiscible phases such as oil and water in a foodstuff. On the basis of their hydrophilic group, there are basically four categories;
- · Anioincs
- · Non- ionics
- · Cationics
- · Amphoterics
Egg is commonly used as an emulsifier. Emulsifier production involves combining oil (triglyceride) with glycerol that results in monoglyceride. The most commonly used raw materials for emulsifiers include palm oil, rapeseed oil, soy bean oil, sunflower oil or lard or tallow.
Emulsifiers are used to assist in fat dispersion, control fat agglomeration, promote air incorporation, enable drier extrusion, increase smoothness and consistency and resist shrinkage on storage.
Milk is a complex colloidal dispersion containing fat globules, casein micelles and whey proteins in an aqueous solution of lactose, minerals and a few other minor compounds. In order to achieve desirable quality attributes in dairy products dairy manufactures have been utilizing ingredients such as stabilizers & emulsifiers.
2. Stabilizers used in dairy industry
2.1. Agar
Agar is a seaweed hydrocolloid or phycocolloid, with long history of use as a gelling, thickening and stabilizing food additive. Agar is a family of linear galactan polysaccaharides obtained from the cellular walls of red seaweeds, Rhodophyceae.
The most important advantages for agar in dairy products derive from the characteristics firm texture and heat tolerance of the gels, stability in acidic conditions and limited reactivity to other food components. The key properties are;
I. It has good compatibility with other polysaccharides and proteins at normal use levels.
This enables agar to give consistent gelled dairy desserts and avoid textural variations which can result from differences in milk quality.
2.2. Alginates
Alginates are derived from brown seaweed and provides a unique combination of properties including cold solubility, cold-setting gels, non-melting gels and freeze-thaw gels.
Alginate is used as a stabiliser, thickener and a gelling agent in dairy products including thickened and canned cream, chocolate mousse, yogurt, bakery creams, milk shake, ice cream and cheese.
Cheese sauce –
· Increased viscosity
· Gives the desired cling needed to stay on food item such as pasta
· Reduce surface skin formation
Ice cream –
· Alginate additions reduce the size of the ice crystals.
· Provides body and a smooth texture.
· Prevents syneresis and delays the meltdown of the ice cream
· In ice cream, calcium ions are naturally present, the alginate is usually combined with a low level of a sodium phosphate, used as a sequestering agent to prevent premature gelling. (fish eyes)
Low- fat butter –
· Alginate is also used in low fat butter to provide the desired texture and to stabilize the emulsion
· Butter are water in oil emulsion, alginate is used to stabilize and weakly gel the water droplets finely distributed in the oil phase.
2.3. Carrageenan
Carrageenan is obtained from red seaweeds harvested around the coasts of the North Atlantic, South America and the Far East.
Low levels of carrageenan, around 100–200 ppm, are used to stabilize and prevent whey separation in a number of dairy products, including milk shake and ice cream mixes, chocolate milks and pasteurized and sterilized creams.
Carrageenan interacts with the dairy proteins to form a network that is able to suspend particulates, such as cocoa in chocolate milk or insoluble calcium salts in calcium fortifies beverages. This network prevent protein-protein interaction and aggregation during storage and avoids whey separation in fluid products and reduce shrinkage in ice cream.
Protein reactivity and gelling properties of carrageenan is used in proceeds chees manufacturing.it gives excellent mouth feel and good melting, grating and slicing properties.
The many dairy products are utilizing the properties of carrageenan;
- · Hot processed milk thickening applications
- · Hot processed milk gelling applications
- · Cold pressed milk thickening applications
2.4. Cellulose Derivatives
Cellulose derivatives are commonly used in food applications where they are effective as viscosifiers, stabilizers and rheology modifiers. It is the main constituent of cell walls of higher plants.
The manufacture of cellulose gum, also known as carboxymethyl cellulose, methyl cellulose, hydroxypropylmethyl (methylhydroxypropyl) cellulose, hydroxypropyl cellulose and ethyl cellulose.
Most cellulose gums may be used to stabilize acidified protein beverages. Because of its anionic nature, cellulose gum will interact with protein. This interaction is used to stabilize protein such as casein at or near the isoelectric point.
Yogurt based drinks requires the use of stabilizer to prevent the precipitation of protein.
Cellulose gum is widely used for ice cream to control the ice crystal growth due its superior performance in heat-shock protection. Cellulose gum appears to be more effective in reducing ice crystal growth in solutions containing proteins.
Also it entrap air and increase overrun of the ice cream. The surface active behavior of cellulose derivatives promotes foam formation during freezing and mixing leading to high overrun products.
Due to the high surface activity, foam stiffness and stability of whipping creams is greatly enhance by using cellulose.
2.5. Gelatine
Gelatine is a proteinaceous material obtained from animal connective tissue using hydrolysis in acidic or basic solution followed by hot water extraction.
Due to the below properties gelatin is able to confer a wide range of benefits to dairy products.
· thermo-reversible gels that are elastic and smooth and melt agreeably in the mouth;
· complete compatibility with milk, casein, other components of milk and principal colloids used in milk products;
· thickening and stabilizing without precipitating casein and without salt addition; binding 5–10 times its weight of water to avoid exudation or syneresis in milk products; acting as a protective colloid so that the coagulation of milk or casein is finer and more homogeneous in the presence of gelatine;
· providing good foaming capacity;
· Providing some emulsification properties.
In full-fat yogurts gelatine improves the texture of the final product obtained by the lactic acid fermentation without modifying the characteristics of taste. Gelatine binds water well and prevents syneresis.
For law-fat yogurts, gelatine give products with a soft texture and firm yoghurts are obtained. Gelatine is particularly useful in the production of fruit yogurts where some syneresis is almost inevitable without the addition of stabilizers. By binding fruit juice, gelatine prevents its diffusion into the yoghurt mass.
The addition of a mixture of gelatine and starch before pasteurization fermented milks, produce a good texture and minimizes any exudation. The texture of a product stabilized with gelatine alone is sensitive to changes in storage temperature.
Gelatine can be used alone or in combination with other gelling agents, such as carrageenan gives a soft, more elastic gel texture for flavored gelled milk desserts. Higher gelatine levels results the preparation of light, aerated products that are very pleasant to eat. And a soft, smooth gel is obtained which has no syneresis.
For dairy dessert creams, gelatine is used to achieve a smooth gel texture and prevent exudation during freezing or as a result of major temperature variations during storage.
Gelatine provide the ice cream with a remarkably slow melting rate and characteristic texture.
Gelatine with alginate, carrageenan or xanthan is use for low fat spreads in order to improve water binding.
Gelatine can be used in cheese production to increase water binding and thus achieve better yield and lower fat content. Gelatine will also strengthen the texture of the product and enhance the flavour release.
2.6. Gellan Gum
Gellan gum is a fermentation polysaccharide produced by the microorganism Sphingomonas elodea.
With its unique and versatile properties, Gellan gum is used commercially in a wide range of dairy food applications.
Typical products include yogurts, sour cream and cheese.
Low- and high-acyl gellan gums can be used in a stirred yogurt, but set yogurts are made only with high-acyl gellan gum. Low-acyl gellan gum creates a lumpy texture after culturing that requires mixing to create a smooth texture. Gellan gum adds a light texture and significantly reduces whey-off.
Sour cream is stabilize with both low- and high-acyl gellan gums.
2.7. Microcrystalline Cellulose (MCC)
Microcrystalline cellulose is purified cellulose produced by converting fibrous cellulose to a redispersible gel or aggregate of crystalline cellulose using acid hydrolysis.
Creams can be stabilized using low-viscosity colloidal MCC. This avoids emulsion separation during storage, particularly under high ambient storage, and contributes to the mouth feel and body of the product. The structure imparted to whipped creams prevents foam drainage and improves stability.
2.8. Pectin
Pectin is a natural constituent of all land plants where, together with cellulose, it plays a key role in the cell wall structure.
In milk products, including yoghurts and fruit and milk desserts, pectin is used as a gelling agent utilizing the natural content of calcium.
Cold-setting milk desserts can be prepared with pectin as a gelling agent. The pectin solution is mixed with cold milk which provides the calcium ions necessary for gelling to take place at low pH. The texture can range from brittle to very soft and creamy, depending on the pectin used.
In stirred and set yoghurt, small amounts of pectin increase firmness, mouth feel and creaminess through excellent water-binding ability, calcium reactivity and interaction with milk proteins. The water binding helps minimize syneresis and the reactivity of pectin with proteins reinforces the protein network in the yoghurt.
3. Emulsifiers used in dairy industry
3.1.Emulsifiers used for ice cream
Ice cream is both a foam and an emulsion, and it contains ice crystals and an unfrozen aqueous phase whose freezing point is depressed by freeze concentration of salts, sugars, and polysaccharide stabilizers.
Emulsifiers that have an improving effect on the structure of ice cream do so because they are able to aid in the destabilization of the milk protein-stabilized ice cream emulsion.
Emulsifiers commonly used in ice cream mix such as glycerol monostearate (GMS) and polysorbates, destabilize the emulsion by displacing protein from the fat-droplet surface. This is a result of their greater surface activity than milk proteins.
3.2. Emulsifiers used for whipped & whipping creams
The terms whipping cream and whipped cream are often used interchangeably, although there are obvious differences between the two both in terms of structure and stability. Whipping cream is an oil-in-water emulsion stabilized by adsorbed milk protein and low molecular weight emulsifiers.
The roles of emulsifiers in whipping cream are;
1. They destabilize the cream through their ability to displace protein from the oil/ water interface. This changes the adsorbed layer composition and interfacial tension of the fat droplet.
2. They may destabilize the emulsion through their ability to form lyotropic liquid crystalline mesophases and the subsequent phase transformations that occur to form stable crystalline forms.
3. They may participate in the initial foam stabilization.
4. They aid in the formation of fat crystals at the fat-droplet surface. These crystals are essential for fat-globule partial coalescence.
3.3. Cheese, processed cheese and cheese products
Processed cheese is a dispersion of fat droplets in a concentrated, gelled protein network.
The main effect of emulsifiers in processed cheese is to increase the solubility of the aggregated cheese proteins through sequestration of calcium, thus improving the emulsifying ability of the caseins. Emulsion stability in the fat droplets is controlled, primarily, by adsorbed caseins or hydrolyzed casein fractions.
Mono- and diglycerides are the emulsifiers adding to processed cheese.
Emulsifiers can have a further effect on the mechanical properties of protein gels when fat droplets are present dispersed throughout the gel network. These so-called emulsion gels are formed when an oil-in-water emulsion is converted to a gel through the action of heat.
3.4. Evaporated and concentrated Milks
Evaporated and concentrated milks are made by removal of water from natural or recombined milks.
Lecithin can be used to increase the heat stability of homogenized and concentrated milks. Lecithin is known to displace protein from the fat-droplet surface and to complex with milk proteins.
3.5. Other Dairy products
Emulsifiers have been added to other dairy products to exploit functional properties not normally associated with such emulsifiers. In recombined butter, phospholipids are added as anti-spitting agents, to prevent fat spitting during heating, and monoglyceride have been claimed to provide better ‘stand-up’ properties during storage.
In addition to being good emulsifiers for use in ice cream, they are known to improve the mouth feel in yoghurt, inhibit microbial growth, enhance the thermal death rate of bacteria and bacterial spores, and increase the heat stability of bovine serum albumin.
It appears that these functions are a result of their ability to bind to proteins.